Why is washing in an ELISA experiment? Is this step so important?
Although washing is not a reaction step, it also determines the success or failure of the experiment. The ELISA relies on washing to achieve the purpose of isolating free and bound enzyme labels. The substance remaining in the pores of the plate which is not capable of binding to the solid phase antigen or antibody, and the interfering substance which is non-specifically adsorbed to the solid phase carrier during the reaction are removed by washing.
Key analysis: two methods of ELISA kit washing
I. Soaking
1. blotting or drying the reaction solution in the well;
2. Wash once with the washing solution (after filling the plate with the washing liquid, it will be removed);
3. Soaking, the washing liquid is filled into the plate hole, placed for 1-2 minutes, intermittent shaking, the soaking time can not be shortened arbitrarily;
4. Drain the liquid in the well. Drying should be thorough, can be pumped by a water pump or a vacuum pump, or can be patted on a clean towel or absorbent paper after removing the liquid;
5. Repeat steps c and d and wash 3-4 times (or as specified). In the indirect method, if the background is higher, the number of washings or the soaking time can be increased.
Second, running water flushing
Originally used for the washing of bead carriers, the washing liquid is only distilled water or even tap water. A special device is attached during washing to continuously roll and wash the beads under the impact of running water. After rinsing for 2 minutes, the liquid is drained, soaked in distilled water for 2 minutes, and blotted dry. The immersion is like a bath, and the running water is like a shower. The washing effect is more thorough and simple and fast. It has been shown in experiments that the flow rinse type is also suitable for the washing of microtiter plates. Try to increase the water flow or increase the water pressure during washing, so that the water flow impacts the surface of the plate hole, and the washing effect is better.
We specialize in manufacturing Microscope prepared slides, medical models, rock specimens, microtome, heating mantles, and lab instruments and consumable materials. New technology update. customization is more diversified. More accurate and longer service life. New changes can better meet the requirements of customer orders. Vic employees consider our company a sign of victory and success. We consider every customer of utmost importance. We consider that we have excellent quality products and service. As a young and enthusiastic team, we will work hard to achieve your needs and requirements.With large stock in our factory, we can cope with large and urgent orders promptly at competitive prices. We make all our efforts to meet your needs with our strong advanges.
Lab Consumable,Lab Consumables List,Laboratory Consumables,Consumables In Laboratory
Xinxiang Vic Science&Education Co.,Ltd. , https://www.hnlaboratoryequipment.com