In the past two decades, immunological analysis methods have developed rapidly, especially after the analysis techniques using labeled antigens and antibodies, so that many of the original analytical methods are incomparable in sensitivity and specificity.
Following the immunofluorescence (IFA) of the 1950s and radioimmunoassay (RIA) analysis techniques of the 1960s, analytical techniques for labeling antigens or antibodies with enzymes were established in the early 1970s. Due to the efficient biocatalysis of the enzyme, an enzyme molecule can catalyze the reaction of tens of hundreds of substrate molecules in a matter of minutes, resulting in amplification, so that the extremely small antigen or antibody can be recognized in a few minutes. This technology is called Enzyme-Linked Immunosorbent Assay (ELISA). This method has been widely reported since the early 1970s by VAN WEEMEN, SCHUARS.ENGVALL and PERLMARN.
ELISA is a new technology in immunodiagnosis. It has been successfully applied to the diagnosis of infectious diseases, parasitic diseases and non-communicable diseases caused by various pathogenic microorganisms. It has also been applied to the quantitative determination of macromolecular antigens and small molecule antigens. According to the results already used, the ELISA method is considered to be sensitive, specific, simple, rapid, stable and easy to automate. It is not only suitable for the examination of clinical specimens, but also for sero-epidemiological investigations because it can check hundreds or even thousands of specimens in one day. This method can be used not only for the determination of antibodies, but also for the determination of circulating antigens in body fluids, so it is also a good method for early diagnosis. Therefore, the application of ELISA in various fields of biomedicine is expanding, and it can be summarized in four aspects:
1. Immune enzyme staining for the localization of various intracellular components.
2. Study the synthesis of anti-enzyme antibodies.
3. A small amount of immunoprecipitation is observed.
4. Quantitative detection of antigen or antibody components in body fluids.
Shanghai Tiancheng Technology Co., Ltd. has a large number of research clients in the national biology and medical circles with its brand name, excellent price and good service. Now, Tiancheng has added a range of diagnostic reagents, a complete warehouse, first-class inspectors, quality administrators, warehouse managers and sales staff to provide you with first-class diagnostic ELISA products. Why did you choose the day:
One. Brand all
ELISA products include human and animal testing series, pet diagnostic reagents, avian disease detection kits, tuberculin, canine disease diagnostic kits, IDS animal diagnostic kits, food safety testing series
After ten years of accumulation, Tiancheng and American RND, American Gaoz, American eBioscience, American BioSource, DSL, American BioCheck, American ADL, American CayMan, American Biovision, American Biovendor, US RB, US MI, US ESBL, American Phoenix, American Enzo (with: biomol, alexis, apo), American GBD, American PPI, American DRG, American RayBiotech, American Millipore, Canada BiOwen, French Diaclone, UK IDS, UK PeProtech, Germany IBL, Germany Weirun Run, Germany, the European Union, Austrian BenDer, Sweden MabTech, the United States IDEXX, France Synbiotics, Germany. In addition, the company has established good and long-term cooperative relationships with several well-known ELISA manufacturers in Beijing, Zhejiang, Shenzhen and Zhejiang.
Second, the price is dominant
Different brands, different boxes have different levels of customers, if you want to graduate, or do pre-experiment, or do research comparison, we have ultra-low price boxes; if you need to publish papers, or develop products We have boxes of world-renowned brands, the same quality, the price of freezing point (especially for peer-to-peer distribution companies). The delivery time varies according to the brand you choose, usually 7-10 days.
Third, supporting equipment
If you have just started an ELISA test or if the instrument is damaged and you want to change the instrument, you are also the best choice.
1. Microplate reader: Tiancheng is the main push Finland Multiskan MK3 Leibo microplate reader (automatic microplate reader): 405nm, 450nm, 492nm, 630nm, BIO-RAD 680 Böhler microplate reader (without printer): 400-750 Mm, standard 4 wavelength, (BIO-TEK) ELX800 Bote microplate reader: 405, 450, 490, 630nm 4 filters. product available,
2. Washing machine: Matching Finnish Multiskan MK3 Regal microplate reader: Finland LABSYSTEMS Wellwash 4 MK2 washing machine; Finland Multiskan MK3 Regal microplate reader matching BIO-RAD 680 Bole labeling instrument for Bole BIO -RAD 1575 Washer; Matching (BIO-TEK) ELX800 Baote Microplate Reader: Baote BIO-TEK ELX50 Washer,
Specific reference: http://?act=product&CategoryID=1708&tagmore=0.
3, refrigerator: 4 ° C refrigerator (reagent freezer), the main push brands are Japan Sanyo, Anhui Meiling, Shandong Haier, reference supermarket website:
Http://?act=product&CategoryID=733&page=1; There are also 37°C incubators, other various sizes of samplers, beakers, test tubes, measuring cylinders, etc.
Fourth, special services
We welcome the close cooperation between the clinical medical community and various research institutes at various levels on various projects to seek development through mutual benefit and common progress, and accumulate experience for the development of China's testing industry.
For any ELISA test kits purchased by the company, you only need to test the types of animals (Human, Rat, Mouse, Rabbit, Monkey, Pig...) and test indicators (intermedias, factors) and specimens (48T). /96T) Notify the company salesperson. On the day of receipt of the customer's specimen, the inspection report will be delivered to your hands within three working days of the spot product!
The following conditions should be noted when sending the specimen:
1. Specimen number; 2. Specimen processing degree; 3. Test item 4, whether to make duplicate holes; 5. Contact method; 6. Whether the specimen is sent back after the experiment
Points to note when collecting specimens:
Before collecting the specimen, it must be clear whether the ingredients to be tested are stable enough. Specimens that were tested on the day after collection should be stored at 4 °C for future use. If there are special reasons, the specimens should be collected periodically, and the specimens should be dispensed in time and stored at -20 °C or -70 °C. Avoid repeated freezing and thawing. Specimens can be stored for 48 hours at 2-8 °C and for 1 month at -20 °C. -70 degrees can be stored for 6 months. Some hormone samples require the addition of aprotinin.
Liquid specimen
Specimens must be liquid and free of sediment. Including serum, plasma, urine, pleural and ascites, cerebrospinal fluid, cell culture supernatant, tissue homogenate and the like. 1 ml of whole blood can give 0.5 ml of serum or plasma. Each specimen volume collection volume = 100 ul x detection type. Ask the salesperson for instructions before taking the material.
serum:
The blood is naturally solidified at room temperature for 10-20 minutes, and then centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant. If a precipitate forms, it should be centrifuged again.
plasma:
EDTA, sodium citrate or heparin should be selected as anticoagulant according to the requirements of the kit. Add 10% (v/v) anticoagulant (0.1M sodium citrate or 1% heparin or 2.0% EDTA.Na2). After 20 minutes, centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms, it should be centrifuged again.
Urine, chest and ascites, cerebrospinal fluid:
Collect with a sterile tube. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms, it should be centrifuged again.
Cell culture supernatant:
When detecting secreted components, collect them in a sterile tube. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. When the components in the cells were detected, the cell suspension was diluted with PBS (pH 7.2-7.4), and the cell concentration reached about 1 million/ml. By repeated freezing and thawing, the cells are destroyed and the intracellular components are released. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again.
Organizational specimen:
After cutting the specimen, weigh the weight. A certain amount of PBS was added, and 1 μg/L protease inhibitor or 50 U/ml Aprotinin (aprotinin) was added to the buffer. The specimen was homogenized by hand or homogenizer. Centrifugation for about 20 minutes (2000-3000 related article: ELISA kit principle; ELISA kit production standard
Infrared thermometers are temperature sensing instruments that are used to make non-contact measurements of surface temperatures. They work by inferring temperatures based on the thermal radiation that is emitted by a particular object. By knowing the object`s emissivity and the amount of infrared energy being emitted, the object`s apparent temperature can then be gauged.
As they are non-contact, they employ the use of a laser to help users aim the thermometer, this is why they are sometimes called laser thermometers. They are commonly used in industrial applications that require quick and accurate temperature readings from a distance when the surface is out of reach, the object`s temperature is too extreme to get near, or when a probe cannot be inserted into the item being measured.
Non-contact body infrared thermometer for professional measurement of human body temperature.The body temperature was measured by collecting infrared thermal radiation from the human frontal hair. It is easy to use and hygienic, simple and quick to operate, and quick and accurate body temperature measurement.Can be widely used in schools, hospitals, homes, ports and other applications.
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