Enantiomeric Separation and Analysis Using Chiral Pesticide by Ultra-High Performance Convergence Chromatography

Enantiomeric Separation and Analysis of Chiral Pesticides in Formulations Using Ultra High Performance Convergence Chromatography (UPC₂) with UV Detection

Marian Twohig¹, Andy Aubin¹, Michael O’Leary¹, Tom DePhillipo², Sherry C. Perine³, and David R. Stubbs³

¹ Waters Corporation (Milford, MA, USA)

² Waters Corporation (Newcastle, DE, USA)

³ Syngenta Crop Protection Corporation (Greensboro, NC, USA)

**Key Benefits of the Method:**

1. This approach enhances the separation of enantiomers and diastereomers compared to traditional normal-phase methods, reduces analysis time, increases sample throughput, and minimizes solvent use.

2. It enables accurate and consistent determination of enantiomeric and diastereomeric ratios in pesticide formulations, ensuring precise application rates and improved safety.

**Background and Importance:**

Agrochemicals play a vital role in reducing crop losses, producing high-quality food, and maintaining global food supply. The goal of agrochemical development is to achieve maximum efficacy with minimal pesticide use.

Approximately 30% of pesticides available today have optical isomers, and over 40% of those used in China are chiral. These chiral compounds often exhibit stereoselective or enantioselective behavior, meaning one enantiomer may be highly active against the target species, while the other may be less effective or even inactive. The less active enantiomer can negatively impact non-target organisms, potentially leading to unintended environmental effects.

To ensure the correct application of active ingredients, it's essential to accurately determine the enantiomeric composition of chiral pesticides. However, separating these stereoisomers presents significant analytical challenges.

While HPLC using chiral stationary phases has been widely used, supercritical fluid chromatography (SFC) offers several advantages, including faster separations and lower solvent consumption. UPC₂ technology, which uses supercritical CO₂ as the primary mobile phase, provides an efficient and complementary approach with orthogonal selectivity to traditional liquid chromatography.

For more details, refer to the attached document: *Enantiomeric Separation and Analysis of Chiral Pesticides in Preparations Using Ultra High Performance Convergence Chromatography (UPC₂) with UV Detector.pdf*

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