A CO₂ incubator is designed to create an environment that closely mimics the conditions found inside a living organism, making it ideal for in vitro cell and tissue culture. It maintains a stable temperature of 37°C, which is the typical body temperature for human cells. The incubator also regulates CO₂ levels at around 5%, which helps maintain the proper pH balance for cell growth. The pH is usually kept within a range of 7.2 to 7.4, similar to the physiological conditions in the body. Additionally, it ensures a high level of relative humidity—typically over 95%—to prevent the culture medium from evaporating and to support optimal cell development. These controlled conditions are essential for maintaining the viability and functionality of cultured cells, allowing researchers to study cellular behavior under consistent and realistic settings. Whether for basic research, drug testing, or biotechnology applications, a CO₂ incubator plays a crucial role in modern life science laboratories.

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