Fluidcm's chief scientific officer Marc Unger once said: "The population average is a lie." Indeed, people gradually realized that no two cells are the same. The study of cell populations can no longer meet our needs, and scientists have begun to analyze the behavior of individual cells. Biocom
With the use of emerging microfluidic methods and molecular biology techniques, the extraction of single cell signals from crowd noise is no longer out of reach. As a leader in the field of single-cell genomics, Fluidigm has been providing researchers with BioMark â„¢ and BioMark HD platforms since 2007, allowing us to easily detect genomic features at the single-cell level. Today, Fluidigm has launched the first single-cell capture and preparation platform on the market, the C1 â„¢ single-cell automatic preparation system.
The C1 system, based on Fluidigm's innovative microfluidic technology, allows researchers to quickly and reliably isolate individual cells and perform genome analysis. Unprecedentedly fully automate the process of separating cells, extracting RNA, reverse transcription and pre-amplified mRNA, making detection and analysis of cell activity possible. Biocom
This system allows you to study cell differentiation, measure individual cell responses to specific stimuli, verify key disease biomarkers, confirm RNAi knockout, and conduct drug screening. With optimized procedures, pre-prepared kits, and disposable chips, you can achieve single-cell accuracy through "load and go".
The workflow of the C1 system is as follows: First, by pipetting, the cell sample in the solution is added to the C1 microfluidic chip. The C1 system will quickly and automatically separate 96 single cells under the input command and distribute them into a single preparation chamber After that, the researchers can choose an internal control point to verify the number of captured cells, distinguish between dead and live cells to maintain the integrity of the data; then, cell lysis, RNA reverse transcription and pre-amplification on the chip, the entire process does not need Any manual solvent mixing and sample transfer operations. Finally, the pre-amplified cDNA products are collected and transferred to the BioMark HD system for quantitative PCR analysis.
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