Our company focuses on product quality, abides by the contract, and has strict requirements for delivery and experimental technology. Therefore, our company has more and more successful customers. In the past five years since the establishment of Shanghai Hengyuan, the teachers’ problems have been summarized, and this has solved the experimental problems for more friends. Are you looking for an answer to these human ELISA kit experiments?
·Why is the standard curve linearly poor?
The standard should be stored as recommended; centrifuge the sample thoroughly before dissolving the standard; ensure that the standard is completely dissolved and mixed, and then follow the serial dilution steps to ensure that each step is thoroughly mixed and accurately pipetted; Appropriate termination of color development; select a suitable mathematical fit equation to draw a standard curve.
·Why should all reagents and test samples be equilibrated to room temperature before loading?
Temperature is an important factor in the ELISA binding response. In order for all samples to react at a consistent temperature, all reagents must be equilibrated to room temperature prior to the experiment, including test samples. Avoid inaccurate ELISA results due to differences in temperature kinetic responses.
· Why do human ELISA kit experiments have to be set up?
In order to obtain more accurate experimental results, it is strongly recommended that the standard and sample be tested for duplicate pores, because the duplicate pore detection can: calculate the average value to ensure more accurate experimental results; solve the jump phenomenon caused by misoperation in the experiment; calculate the CV value, The operation of the experiment and the precision of the kit were evaluated.
· Poor repeatability For this, it is likely to be:
1 The number of samples varies, and the loading time varies. (Constantly solved: When repeating a sample, the loading time is as close as possible to the first time).
3 The microplate reader filter is wrong or the input wavelength is not correct (constantly solved: TMB color is used for 450/630 wavelength).
4 The microplate reader measures poor reproducibility (constantly solved by Hengyuan: proofreading microplate reader).
Today's information content is here, my company's human ELISA kit experiments are safe and reliable, the specifications are 96T and 48T two, the main agent brands are the United States very well-known R & D, TSZ.
·Why is the standard curve linearly poor?
The standard should be stored as recommended; centrifuge the sample thoroughly before dissolving the standard; ensure that the standard is completely dissolved and mixed, and then follow the serial dilution steps to ensure that each step is thoroughly mixed and accurately pipetted; Appropriate termination of color development; select a suitable mathematical fit equation to draw a standard curve.
·Why should all reagents and test samples be equilibrated to room temperature before loading?
Temperature is an important factor in the ELISA binding response. In order for all samples to react at a consistent temperature, all reagents must be equilibrated to room temperature prior to the experiment, including test samples. Avoid inaccurate ELISA results due to differences in temperature kinetic responses.
· Why do human ELISA kit experiments have to be set up?
In order to obtain more accurate experimental results, it is strongly recommended that the standard and sample be tested for duplicate pores, because the duplicate pore detection can: calculate the average value to ensure more accurate experimental results; solve the jump phenomenon caused by misoperation in the experiment; calculate the CV value, The operation of the experiment and the precision of the kit were evaluated.
· Poor repeatability For this, it is likely to be:
1 The number of samples varies, and the loading time varies. (Constantly solved: When repeating a sample, the loading time is as close as possible to the first time).
3 The microplate reader filter is wrong or the input wavelength is not correct (constantly solved: TMB color is used for 450/630 wavelength).
4 The microplate reader measures poor reproducibility (constantly solved by Hengyuan: proofreading microplate reader).
Today's information content is here, my company's human ELISA kit experiments are safe and reliable, the specifications are 96T and 48T two, the main agent brands are the United States very well-known R & D, TSZ.
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