Problems that should be noted when using ELISA kits
1. The experiment is carried out in strict accordance with the operation of the manual. The determination of the experimental results must be based on the reading of the microplate reader.
2. If the enzyme label is not used up after being opened, it should be immediately placed in a sealed bag and desiccant.
3. It is recommended that all standards, samples and blanks be double-tested and averaged to reduce experimental error.
4. Keep in mind that the sample has been diluted 5 times and the calculated result is multiplied by 5 to determine the actual concentration of the sample.
5. The quantitative range of this kit is 0.78-50ng/L. If it exceeds this range, it is calculated by the extension of the standard curve. It is not used as an accurate quantitative result. Please dilute with special dilution solution to determine the accurate result (0.78-50ng/L). ), multiplied by the total dilution factor is the final concentration of the sample.
6. If the color is too light, the substrate incubation time can be extended appropriately.
7. In order to avoid cross-contamination, the standard product, sample and blank control should be replaced once for each additional one; the common components such as enzyme standard working solution, sample diluent and substrate should be cantilevered and should not touch micropores. ; Do not reuse the sealing film.
8. The kit is used during the shelf life. The reagents of different batches must not be mixed.
9. Substrate B is sensitive to light and avoids prolonged exposure to light.
Office Staples,Staples Coupons,Staples Hyken,Staples Binders
SHAOXING YIYOU STATIONERY CO.,LTD , https://www.yiyou-staple.com